Journal: Journal of medicinal chemistry

Article Title: From DNA-Encoded Library Screening to AM-9747 : An MTA-Cooperative PRMT5 Inhibitor with Potent Oral In Vivo Efficacy.

doi: 10.1021/acs.jmedchem.4c03101

Figure Lengend Snippet: Figure 7. (A) AM-9747 viability in HCT116 cellular assay (blue circles, MTAP-WT; red squares MTAP-del). Viability was measured by a CellTiter-Glo assay, and cellular MTA-selectivity was determined as (HCT116-WT IC50/MTAP-del IC50). (B) HCT116-WT and MTAP-del global SDMA levels were assessed by an in-cell imaging assay after 3 days of treatment with AM-9747.

Article Snippet: Cells were then stained with an anti-SDMA polyclonal antibody (CST#13222S at 1:2000 dilution) in a wash buffer and incubated at RT for 2 h. Cells were washed with 200 mL of Wash Buffer and stained with secondary antibodies (goat antirabbit-alexa-488 Cat #A11034, Invitrogen at 1:2000 dilution) and Hoechst 33342 DNA dye (1:5000 dilution) for 1 h at RT in the dark.

Techniques: Glo Assay, Imaging